Journal
MICROBIAL BIOTECHNOLOGY
Volume 15, Issue 9, Pages 2439-2449Publisher
WILEY
DOI: 10.1111/1751-7915.14074
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Funding
- Nanjing Medical University Fan Daiming Research Funds for Holistic Integrative Medicine
- National Natural Science Foundation of China [81670495, 81873548]
- China National Center for Clinical Research of Digestive Diseases [2015BAI13B07]
- Intestine Initiative
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This study describes the methodology development of faecal microbiota transplantation (FMT) in China from manual to washing preparation, and provides insights into donor screenings, treatment doses, adverse events and delivery decisions. The success rate of donor screening was found to be 3.1%, and the incidence rate of fever decreased significantly with washed microbiota transplantation (WMT) in ulcerative colitis patients. The study also suggests a new definition for treatment units based on enriched microbiota and proposes a promising method for delivering microbiota through colonic transendoscopic enteral tube.
The safety, quantitative method and delivery of faecal microbiota transplantation (FMT) vary a lot from different countries in practice. Recently, the improved methodology of FMT based on the automatic filtration, washing process and the related delivery was named as washed microbiota transplantation (WMT). First, this study aimed to describe the methodology development of FMT from manual to washing preparation from 2012 to 2021 in China Microbiota Transplantation System (CMTS), a centralized stool bank for providing a national non-profit service. The secondary aim is to describe donor screenings, the correlation between faecal weight and treatment doses, incidence of adverse events and delivery decision. The retrospective analysis on the prospectively recorded data was performed. Results showed that the success rate of donor screening was 3.1% (32/1036). The incidence rate of fever decreased significantly from 19.4% (6/31) in manual FMT to 2.7% (24/902) in WMT in patients with ulcerative colitis (UC), which made UC a considerable disease model to reflect the quality control of faecal microbiota preparation. We defined one treatment unit as 10 cm(3) microbiota precipitation (1.0 x 10(13) bacteria) based on enriched microbiota instead of rough faecal weight. For delivering microbiota, colonic transendoscopic enteral tube is a promising way especially for multiple WMTs or frequent colonic administration of drugs combined with WMT. This study should help improve the better practice of FMT for helping more patients in the future.
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