Journal
CELL DISCOVERY
Volume 8, Issue 1, Pages -Publisher
SPRINGERNATURE
DOI: 10.1038/s41421-022-00385-3
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Funding
- National Natural Science Foundation of China [U21A200029]
- National Key R&D Program of China [2019YFA0802202]
- 111 Project [B13026]
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The study reveals that the m(1)A demethylase ALKBH3 inhibits ciliogenesis by removing m(1)A sites on Aurora A mRNA. Depletion of ALKBH3 leads to ciliary defects in zebrafish embryos, which can be rescued by wild-type ALKBH3. These findings highlight the crucial role of ALKBH3-dependent m(1)A demethylation in ciliogenesis and developmental events.
Primary cilia are antenna-like subcellular structures to act as signaling platforms to regulate many cellular processes and embryonic development. m(1)A RNA modification plays key roles in RNA metabolism and gene expression; however, the physiological function of m(1)A modification remains largely unknown. Here we find that the m(1)A demethylase ALKBH3 significantly inhibits ciliogenesis in mammalian cells by its demethylation activity. Mechanistically, ALKBH3 removes m(1)A sites on mRNA of Aurora A, a master suppressor of ciliogenesis. Depletion of ALKBH3 enhances Aurora A mRNA decay and inhibits its translation. Moreover, alkbh3 morphants exhibit ciliary defects, including curved body, pericardial edema, abnormal otoliths, and dilation in pronephric ducts in zebrafish embryos, which are significantly rescued by wild-type alkbh3, but not by its catalytically inactive mutant. The ciliary defects caused by ALKBH3 depletion in both vertebrate cells and embryos are also significantly reversed by ectopic expression of Aurora A mRNA. Together, our data indicate that ALKBH3-dependent m(1)A demethylation has a crucial role in the regulation of Aurora A mRNA, which is essential for ciliogenesis and cilia-associated developmental events in vertebrates.
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