Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice

Specific gene and miRNA expression patterns are potential early biomarkers of harmful environmental carcinogen exposures. The aim of our research was to develop an assay panel by using several miRNAs for the rapid screening of potential carcinogens. The expression changes of miR-124-1, miR-212, miR-132, miR-134, and miR-155 were examined in the spleen, liver, and kidneys of CBA/Ca mice, following the 20 mg/bwkg intraperitoneal 7,12-dimethylbenz(a)anthracene (DMBA) treatment. After 24 h RNA was isolated, the miRNA expressions were analyzed by a real-time polymerase chain reaction and compared to a non-treated control. DMBA induced significant changes in the expression of miR-134, miR-132, and miR-124-1 in all examined organs in female mice. Thus, miR-134, miR-132, and miR-124-1 were found to be suitable biomarkers for the rapid screening of potential chemical carcinogens and presumably to monitor the protective effects of chemopreventive agents.

Automated summary

Specific gene and miRNA expression patterns can serve as potential early biomarkers for harmful environmental carcinogen exposures. This research aimed to develop an assay panel using several miRNAs for the rapid screening of potential carcinogens. The study examined the expression changes of miR-124-1, miR-212, miR-132, miR-134, and miR-155 in the spleen, liver, and kidneys of mice following DMBA treatment. The results showed significant changes in the expression of miR-134, miR-132, and miR-124-1, suggesting their suitability as biomarkers for screening potential chemical carcinogens and monitoring the protective effects of chemopreventive agents.