Journal
CELLS
Volume 11, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/cells11081337
Keywords
keratinocytes; vaccinia virus; atopic dermatitis; barrier disruption; type 2 cytokines
Categories
Funding
- National Institute of Allergy and Infectious Diseases [AI146800, AI126005, U01AI152011, AI067691, T32 AI18689, T32 AI007285, 1U19AI117673]
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Individuals with atopic dermatitis (AD) are more susceptible to viral infections, but little is known about how the inflammatory environment affects the susceptibility of skin cells to viruses. This study found that under conditions simulating AD, keratinocytes become more susceptible to vaccinia virus (the causative agent of eczema vaccinatum), and this susceptibility can be reduced by inhibiting JAK.
Individuals with underlying chronic skin conditions, notably atopic dermatitis (AD), are disproportionately affected by infections from members of the herpesviridae, papovaviridae, and poxviridae families. Many patients with AD experience recurrent, widespread cutaneous viral infections that can lead to viremia, serious organ complications, and even death. Little is known about how the type 2 inflammatory environment observed in the skin of AD patients impacts the susceptibility of epidermal cells (keratinocytes) to viral pathogens. Herein, we studied the susceptibility of keratinocytes to the prototypical poxvirus, vaccinia virus (VV)-the causative agent of eczema vaccinatum-under conditions that simulate the epidermal environment observed in AD. Treatment of keratinocytes with type 2 cytokines (IL-4 and -13) to simulate the inflammatory environment or a tight junction disrupting peptide to mirror the barrier disruption observed in AD patients, resulted in a differentiation-dependent increase in susceptibility to VV. Furthermore, pan JAK inhibition was able to diminish the VV susceptibility occurring in keratinocytes exposed to type 2 cytokines. We propose that in AD, the increased viral susceptibility of keratinocytes leads to enhanced virus production in the skin, which contributes to the rampant dissemination and pathology seen within patients.
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