Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex

At glutamatergic synapses, astrocytes respond to the neurotransmitter glutamate with intracellular Ca2+ elevations and the release of gliotransmitters that modulate synaptic transmission. While the functional interactions between neurons and astrocytes have been intensively studied at glutamatergic synapses, the role of astrocytes at GABAergic synapses has been less investigated. In the present study, we combine optogenetics with 2-photon Ca2+ imaging experiments and patch-clamp recording techniques to investigate the signaling between Somatostatin (SST)-releasing GABAergic interneurons and astrocytes in brain slice preparations from the visual cortex (VCx). We found that an intense stimulation of SST interneurons evokes Ca2+ elevations in astrocytes that fundamentally depend on GABA(B) receptor (GABA(B)R) activation, and that this astrocyte response is modulated by the neuropeptide somatostatin. After episodes of SST interneuron hyperactivity, we also observed a long-lasting reduction of the inhibitory postsynaptic current (IPSC) amplitude onto pyramidal neurons (PNs). This reduction of inhibitory tone (i.e., disinhibition) is counterbalanced by the activation of astrocytes that upregulate SST interneuron-evoked IPSC amplitude by releasing ATP that, after conversion to adenosine, activates A(1)Rs. Our results describe a hitherto unidentified modulatory mechanism of inhibitory transmission to VCx layer II/III PNs that involves the functional recruitment of astrocytes by SST interneuron signaling.

Automated summary

In this study, optogenetics and 2-photon Ca2+ imaging experiments were used to investigate the signaling between Somatostatin (SST)-releasing GABAergic interneurons and astrocytes in brain slice preparations from the visual cortex. It was found that intense stimulation of SST interneurons evokes Ca2+ elevations in astrocytes depending on GABA(B) receptor activation, with this response modulated by the neuropeptide somatostatin. Additionally, after episodes of SST interneuron hyperactivity, a long-lasting reduction of inhibitory postsynaptic current (IPSC) onto pyramidal neurons was observed, which was counterbalanced by the activation of astrocytes that upregulate SST interneuron-evoked IPSC amplitude through the release of ATP and conversion to adenosine.