4.6 Article

Elucidation of Focal Adhesion Kinase as a Modulator of Migration and Invasion and as a Potential Therapeutic Target in Chronic Lymphocytic Leukemia

Journal

CANCERS
Volume 14, Issue 7, Pages -

Publisher

MDPI
DOI: 10.3390/cancers14071600

Keywords

chronic lymphocytic leukemia; FAK; TLR9; migration; transcriptomics; miRNomics; microenvironment

Categories

Funding

  1. Blood Cancer UK Programme Continuity Grant [18005]
  2. MRC [MR/V009095/1]
  3. Sussex Cancer Fund
  4. Crawley Leukaemia Research
  5. Michael Chowen Research Fund
  6. Leukaemia UK John Goldman Fellowship [2020/JGF/003]
  7. UKRI Future Leaders Fellowship [MR/T041889/1]
  8. MRC [MR/V009095/1] Funding Source: UKRI

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The study revealed the role of the FAK signaling pathway in CLL cell migration and tissue invasion by investigating the mechanisms underlying CLL cell migration. Inhibition of FAK effectively reduced CLL cell migration and invasion, suggesting the potential for combining FAK inhibition with current targeted therapies to enhance CLL treatment efficacy.
Simple Summary Despite the successful introduction of targeted therapies, Chronic Lymphocytic Leukemia (CLL) remains incurable. This is thought to be partially due to the pro-survival and anti-apoptotic signaling that CLL cells receive from the lymph node microenvironment. Therefore, inhibition of CLL migration into the lymph nodes is an attractive therapeutic option. Here, our aim was to gain a further understanding of what transcriptomic and miRNomic changes drive CLL migration and, from this, select promising therapeutic targets. We identified focal adhesion kinase (FAK) as one such potential target and demonstrated that inhibition of FAK in primary CLL samples effectively reduces both CXCL12 induced migration and invasion in vitro. Successful inhibition of CLL migration could increase the sensitivity of CLL cells to the currently used targeted therapeutics and therefore improve patient outcomes. The retention and re-migration of Chronic Lymphocytic Leukemia cells into cytoprotective and proliferative lymphoid niches is thought to contribute to the development of resistance, leading to subsequent disease relapse. The aim of this study was to elucidate the molecular processes that govern CLL cell migration to elicit a more complete inhibition of tumor cell migration. We compared the phenotypic and transcriptional changes induced in CLL cells using two distinct models designed to recapitulate the peripheral circulation, CLL cell migration across an endothelial barrier, and the lymph node interaction between CLL cells and activated T cells. Initially, CLL cells were co-cultured with CD40L-expressing fibroblasts and exhibited an activated B-cell phenotype, and their transcriptional signatures demonstrated the upregulation of pro-survival and anti-apoptotic genes and overrepresentation of the NF-kappa B signaling pathway. Using our dynamic circulating model, we were able to study the transcriptomics and miRNomics associated with CLL migration. More than 3000 genes were altered when CLL cells underwent transendothelial migration, with an overrepresentation of adhesion and cell migration gene sets. From this analysis, an upregulation of the FAK signaling pathway was observed. Importantly, PTK2 (FAK) gene expression was significantly upregulated in migrating CLL cells (PTK2 Fold-change = 4.9). Here we demonstrate that TLR9 agonism increased levels of p-FAK (p <= 0.05), which could be prevented by pharmacological inhibition of FAK with defactinib (p <= 0.01). Furthermore, a reduction in CLL cell migration and invasion was observed when FAK was inhibited (p <= 0.0001), supporting a role for FAK in both CLL migration and tissue invasion. When taken together, our data highlights the potential for combining FAK inhibition with current targeted therapies as a more effective treatment regime for CLL.

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