Journal
CANCERS
Volume 14, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/cancers14071801
Keywords
lung cancer; CD47; SIRP alpha; CD68; PD-L1; FOXP3; prognosis
Categories
Funding
- project InTechThrace: Integrated Technologies in biomedical research: multilevel biomarker analysis in Thrace under the Operational Program Competitiveness, Entrepreneurship & Innovation (EPAnEK) [5047285]
- European Regional Development Fund (ERDF)
- European Union (European Social Fund- ESF)
- Democritus University of Thrace Special Account [81006]
- MSc course of Translational Research in Biomedicine (Department of Molecular Biology and Genetics)
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The study showed that high CD47 expression by cancer cells, along with high SIRPα expression by CD68+ TAMs, low TIL-score, and poor prognosis are correlated in NSCLC. CD68+ TAMs play an important role in the prognosis of operable NSCLC. Targeting the CD47/SIRPα axis may improve adjuvant immunotherapy strategies in NSCLC.
Background: Cancer cells escape macrophage phagocytosis by expressing the CD47 integrin-associated protein that binds to the SIRP alpha ligand (signal regulatory protein alpha) expressed by macrophages. Immunotherapy targeting this pathway is under clinical development. Methods: We investigated the expression of CD47/SIRP alpha molecules in a series of 98 NSCLCs, in parallel with the infiltration of tumor stroma by CD68+ macrophages, tumor-infiltrating lymphocytes (TILs), and PD-L1/PD-1 molecules. Results: Extensive membranous CD47 expression by cancer cells characterized 29/98 cases. SIRP alpha and CD68 were expressed, to a varying extent, by tumor-associated macrophages (M phi, TAMs). A high CD68M phi-score in inner tumor areas was linked with improved overall survival (p = 0.005); and this was independent of the stage (p = 0.02, hazard ratio 0.4). In contrast, high SIRP alpha expression by CD68+ TAMs (SIRP alpha/CD68-ratio) was linked with CD47 expression by cancer cells, low TIL-score, and poor prognosis (p = 0.02). A direct association of CD47 expression by cancer cells and the % FOXP3+ TILs (p = 0.01, r = 0.25) was also noted. Conclusions: TAMs play an important role in the prognosis of operable NSCLC. As SIRP alpha+ macrophages adversely affect prognosis, it is suggested that the CD47/SIRP alpha+ axis is a sound target for adjuvant immunotherapy policies, aiming to improve the cure rates in operable NSCLC.
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