Journal
JOURNAL OF CLINICAL MEDICINE
Volume 11, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/jcm11071824
Keywords
ovarian aging; in vitro fertilization; pre-implantation genetic testing for aneuploidy; mitochondrial DNA copy number; telomere length
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Funding
- Chung Shan Medical University Hospital [CSH-2019-C-034]
- Ministry of Science and Technology, Taiwan [MOST 298 106-3114-B-040-001]
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The present study aims to investigate the correlation among female age, cellular aging markers, and aneuploidy rate. The results demonstrate that telomere length (TL) in granulosa cells is negatively correlated with the aneuploidy rate in the young-age group.
Background: To identify the correlation among female age, cellular aging markers, and aneuploidy rate in in vitro fertilization (IVF) and the preimplantation genetic test for aneuploidy (PGT-A) cycles. Methods: This is a prospective cohort study recruiting 110 infertile women between August 2017 and July 2018. They were divided into young-age (<38 years, n = 60) and advanced-age (>= 38 years, n = 50) groups. Peripheral leukocytes were assessed, and the granulosa cells were pooled during oocyte pickup. Mitochondrial DNA (mtDNA) copy number and telomere length (TL) were measured using real-time polymerase chain reaction. PGT-A was performed on the NGS platform. Results: mtDNA copy number and TL were positively correlated in both leukocytes (rho = 0.477, p < 0.001) and granulosa cells (rho = 0.361, p < 0.001), but the two parameters in leukocytes were not correlated with those in granulosa cells. In the young-age group, TL in the granulosa cells was the only factor correlated with the aneuploidy rate (rho = -0.283, p = 0.044), whereas in the advanced-age group, age was the main factor (rho = 0.358, p = 0.018). Conclusions: TL in the granulosa cells was negatively correlated with the aneuploidy rate in the young-age group, supporting the application of PGT-A in younger women.
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