Unravelling the Molecular Interaction of Pentoxifylline with Calf Thymus DNA: A Multitechnique Approach

The drug-DNA interaction studies provide vital insights for designing and developing effective chemotherapeutic agents used in treatment of different cancers. Thus, the interaction of potential anticancer agent pentoxifylline and calf thymus DNA was analysed by various spectroscopic and electrochemical methods under physiological condition. The absorption spectra of pentoxifylline with calf thymus DNA revealed the formation of complex by hyperchromic effect with a binding constant of 1.461 x10(4) M-1. Fluorescence studies, further showed that pentoxifylline binds to calf thymus DNA and form a stable complex with a static quenching mechanism. Thermodynamic analysis further confirms that the interaction process is spontaneous with Vander Waals force or hydrogen bonding playing a major role. The electrochemical studies and circular dichroism spectra signify that pentoxifylline interacts by groove binding mode. Based on electrochemical and spectroscopic results, the mode of pentoxifylline binding to calf thymus DNA is mainly established through groove binding.

Automated summary

The drug-DNA interaction studies are important for the design and development of effective chemotherapeutic agents. This study found that pentoxifylline interacts with calf thymus DNA through a stable complex formation, mainly via hyperchromic effect and static quenching mechanism.