Journal
JOURNAL OF BIOTECHNOLOGY
Volume 218, Issue -, Pages 108-114Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2015.12.004
Keywords
Astaxanthin; Microalgal biomass; Enzymatic lysis; Microencapsulation; SEDS
Categories
Funding
- CNPq
- CAPES
- FAPERGS
- NANOFOTO-BIOTEC
Ask authors/readers for more resources
In this work, the effectiveness of different enzymatic techniques for cell wall disruption of Haematococcus pluvialis for the extraction of carotenoids and subsequent encapsulation of extracts in the co-polymer poly(hydroxybutyrate-co-hydroxyvalerate)(PHBV) using the Solution Enhanced Dispersion by Supercritical fluids (SEDS) technique was investigated. Glucanex (R) performed best compared with Lyticase (R) and Driselase (R). The conditions for enzymatic lysis using this enzyme preparation were established as a pH of 4.5, a temperature of 55 degrees C, an initial activity of beta-1,3-glucanase of 0.6 U mL(-1) and a reaction time of 30 min. Enzymatic lysis assisted by ultrasound without biomass freezing was shown to be a promising and simple one-step technique for cell wall disruption, reaching 83.90% extractability. In the co-precipitation experiments, the highest encapsulation efficiency (51.21%) was obtained when using a higher biomass to dichloromethane ratio (10 mg mL(-1)) at the carotenoid extraction step and a lower pressure of precipitation (80 bar). In these conditions, spherical particles in the micrometer range (0.228 mu m) were obtained. (C) 2015 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available