Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum

The water-soluble polysaccharide LBP-1 was isolated and characterized from Lycium barbarum L. LBP-1 was mainly composed of arabinose, galactose, glucose, xylose, mannose at a molar ratio of 37.53: 28.08: 14.72: 7.83: 4.50, respectively. Based on nuclear magnetic resonance (NMR) and methylation analysis, the backbone of LBP-1 was speculated in the alpha-L-Ara(1 ->[5-alpha-L-Ara(1 -> 3)-8-D-Galp-(1 ->]n -> 4)-alpha-D-Galp-(1[-> 5-alpha-L-Ara (1]n [-> 6)8-D-Galp-(1 -> 4)-8-D-Galp-(1 ->]n, and the side chains of LBP-1 were in the alpha-L-Ara(1 -> 3)-8-D-Galp-(1 -> 6 position. These results showed that LBP-1 inhibited the growth of cancer A549 cells through cell cycle arrest and apoptosis, with an IC50 value of 42.5 mu g/mL. In addition, LBP-1 altered the expression of Cyclin D1, Cyclin D3, and CDK 2, thus blocking the cell cycle in G0/G1 phase, reducing cell migration, and regulating the PI3K/Akt/ mTOR signaling pathway to induce apoptosis.

Automated summary

A water-soluble polysaccharide LBP-1 was isolated from Lycium barbarum L. and found to inhibit the growth of cancer cells through cell cycle arrest and apoptosis.