Journal
JOURNAL OF BIOTECHNOLOGY
Volume 237, Issue -, Pages 1-12Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2016.08.018
Keywords
srf genes knockout; Bacillus subtilis PB2-LS10; Transcriptome
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Funding
- National Natural Science Foundation of China [31271828]
- National Science and Technology Support program [2011BAD23B05]
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Gene knockout is an important approach to improve the production of antimicrobial compounds. B. subtilis PB2-LS10, derived from B. subtilis PB2-L by a surfactin synthetase (srf) genes knockout, exhibits stronger inhibitory action than its parental strain against all tested pathogenic bacteria and fungi. The antimicrobial extracts produced by B. subtilis PB2-L and B. subtilis PB2-LS10 respectively were characterized by the high-resolution LC-ESI-MS. To provide further insight into the distinct antimicrobial activities, we investigated the impact of the srf genes deletion on the growth and gene transcriptional profile of the strains. The mutant strain grew quickly and reached stationary phase 2 h earlier than the wild-type. Prominent expression changes in the modified strain involved genes that were essential to metabolic pathways and processes. Genes related to amino acid transport, ATP-binding cassette (ABC) transporters and protein export were up-regulated in strain PB2-LS10. However, amino acid metabolism, carbohydrate metabolism and fatty acid metabolism were repressed. Because of its excellent antimicrobial activity, strain PB2-LS10 has potential for use in food preservation. (C) 2016 Elsevier B.V. All rights reserved.
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