Journal
JOURNAL OF BIOTECHNOLOGY
Volume 227, Issue -, Pages 27-34Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2016.03.038
Keywords
Asymmetric transposon; Codon deletions; Mutant library; Green fluorescent protein; Reading frame selection
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Funding
- ShanghaiTech University
- University of Maryland, College Park
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Substitutions, insertions and deletions are all important mutation events in natural and laboratory protein evolution. However, protein engineering using insertions and deletions (indels) is hindered by the lack of a convenient mutagenesis method. Here, we describe a general transposon mutagenesis method that allows for removal of up to five consecutive in-frame codons from a random position of a target protein. This method, referred to as codon deletion mutagenesis (CDM), relies on an engineered Mu transposon that carries asymmetric terminal sequences flanking the MuA transposase recognition sites. CDM requires minimal DNA manipulations, and can generate multi-codon deletions with high efficiency (>90%). As a proof of principle, we constructed five libraries of green fluorescent protein (GFP) containing one to five random codon deletions, respectively. Several variants with multi-codon deletions remained fluorescent, none of which could be easily identified using traditional mutagenesis method. CDM provides a facile and efficient approach to sampling a protein sequence with multi-codon deletions. It will not only facilitate our understanding of the effects of amino acid deletions on protein function but also expedite protein engineering using deletion mutagenesis. (C) 2016 Elsevier B.V. All rights reserved.
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