Partitional Behavior of Janus Dumbbell Microparticles in a Polyethylene Glycol (PEG)-Dextran (DEX) Aqueous Two-Phase System (ATPS)

Janus particles are known to be useful to various fields such as biomolecule-probing sensors, reaction catalysts, surfactants, and so on. They have two chemically different surfaces which possess contradictory characteristics such as polarity, hydrophobicity, etc. Here, a simple fabrication of dumbbell-shaped Janus microparticles was tested by the chemical reaction of carboxyl groups and amino groups to form amide bonds. They were distributed to the interface between polyethylene glycol (PEG)-rich phase and dextran (DEX)-rich phase, while the unreacted particles having carboxyl groups located at the top PEG-rich phase and particles having amine ligands went to the bottom DEX-rich phase of an aqueous two-phase system (ATPS). The fabrication procedures, observations, and possible applications of results are discussed.

Automated summary

Janus particles with two chemically different surfaces, useful in various fields, were fabricated by forming amide bonds through a chemical reaction and distributed in an aqueous two-phase system. The particles with carboxyl groups located at the top phase, and those with amine ligands went to the bottom phase, showing potential applications in biomolecule-probing sensors and reaction catalysts.