Journal
JOURNAL OF BIOTECHNOLOGY
Volume 221, Issue -, Pages 62-69Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2016.01.022
Keywords
Selective oxidation; Corynebacterium sp.; Trimethylolpropane; 2,2-Bis(hydroxymethyl)butyric acid; Whole cell biotransformation; Product recovery
Categories
Funding
- Cultural affairs and mission sector in Egyptian ministry of higher education
- Swedish Research Council Formas for Environment, Agricultural Sciences and Spatial Planning
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Multifunctional chemicals including hydroxycarboxylic acids are gaining increasing interest due to their growing applications in the polymer industry. One approach for their production is a biological selective oxidation of polyols, which is difficult to achieve by conventional chemical catalysis. In the present study, trimethylolpropane (TMP), a trihydric alcohol, was subjected to selective oxidation using growing cells of Corynebacterium sp. ATCC 21245 as a biocatalyst and yielding the dihydroxy-monocarboxylic acid, 2,2-bis(hydroxymethyl) butyric acid (BHMB). The study revealed that co-substrates are crucial for this reaction. Among the different evaluated co-substrates, a mixture of glucose, xylose and acetate at a ratio of 5: 5: 2 was found optimum. The optimal conditions for biotransformation were pH 8, 1 v/v/m airflow and 500 rpm stirring speed. In batch mode of operation, 70.6% of 5 g/l TMP was converted to BHMB in 10 days. For recovery of the product the adsorption pattern of BHMB to the anion exchange resin, Ambersep (R) 900 (OH-), was investigated in batch and column experiments giving maximum static and dynamic binding capacities of 135 and 144 mg/g resin, respectively. BHMB was separated with 89.7% of recovery yield from the fermentation broth. The approach is applicable for selective oxidation of other highly branched polyols by biotransformation. (C) 2016 Elsevier B.V. All rights reserved.
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