Journal
JOURNAL OF BIOTECHNOLOGY
Volume 236, Issue -, Pages 71-77Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2016.08.009
Keywords
Haematococcus pluvialis; Astaxanthin; Three-stage mutagenesis; Diphenylamine
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Funding
- 12th Five-year-plan in National Science and Technology for the Rural Development Project of China [2013BAD10B02-06]
- Development of Science and Technology Project of Shandong Province [2014GSF121029]
- National Natural Science Foundation of China [31471657]
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Haematococcus pluvialis was modified for higher astaxanthin production compatible with the superiorities of high biomass and high activity by three-stage mutagenesis breeding. UV irradiation mutants named UV11-4 made an increase on cell dry weight, but showed a longer growth circle than the wild type. On the basis of UV mutants, ethyl methane sulphonate (EMS) mutants E2-5 cut down the latent phase, brought forward and extended the logarithmic phase. The inhibitor diphenylamine (DPA) was employed to screen high-yield astaxanthin producer by the color change of colonies from green to red on solid medium. Via the contravariant cultivation, proliferation and transformation, the mutant DPA12-2 possessed an 1.7-fold astaxanthin production compared to the wild type, reaching 47.21 +/- 3.30 mg/g dry cells. (C) 2016 Elsevier B.V. All rights reserved.
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