Journal
JOURNAL OF BIOTECHNOLOGY
Volume 240, Issue -, Pages 76-84Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2016.11.001
Keywords
Bioactive peptides; Hypertension; Spontaneous hypertensive rats; Microalgae
Categories
Funding
- FCQ-UASLP
- [CONACYT CB-15148]
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In this study, we developed a transplastomic C. reinhardtii strain that accumulates anti-hypertensive peptides. Tandem repeats of VLPVP peptide were included. PCR analysis confirmed the presence of the transgene in the modified strains. After in vitro digestion of biomass of a recombinant C. reinhardtii strain the VLVPV peptide was identified and quantified by HPLC. The highest expression line produced 0.292 mg of recombinant protein per mg of freeze-dried biomass. Intragastric administration of the genetically modified strain to spontaneous hypertensive rats at a dose of 30 mg/kg of body weight of recombinant protein significantly reduced systolic blood pressure. At the same dose, the recombinant protein exerts an ACE-inhibitory effect. This is the first study that indicates the potential of this microalga producing an antihypertensive peptide as a dietary supplement for hypertension patients. (C) 2016 Elsevier B.V. All rights reserved.
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