Journal
SCIENCE CHINA-MATERIALS
Volume 65, Issue 10, Pages 2861-2870Publisher
SCIENCE PRESS
DOI: 10.1007/s40843-022-2008-1
Keywords
diacetylene; lipidated peptide amphiphile; electrostatic interaction; reactive oxygen species; in-cellulo polymerization
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Funding
- National Natural Science Foundation of China [52173124]
- Fundamental Research Funds for the Central Universities [2172019kfyXJJS070]
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This study presents a designed peptide micelle capable of undergoing in-situ polymerization inside cells, enabling cell labeling and long-term tracking using fluorescence. The polymerization process induces tumor cell apoptosis, increases cell viscosity, and decreases cell motility, potentially inhibiting tumor metastasis.
Recently, in-situ polymerization inside living cells has attracted much attention due to the efficient cellular internalization and elevated drug retention. However, the lack of tracking of the in-situ polymerization process and the unclear effects of polymerization on cellular functions restrict its biomedical applications. Herein, we designed a Y-shaped diacetylene-containing lipidated peptide amphiphile (Y-DLPA1) with positive charges, which underwent in-situ polymerization initiated by reactive oxygen species in the intracellular microenvironment. In comparison, zwitterionic Y-DLPA2 and negatively charged Y-DLPA3 were polymerized in aqueous solution, but cannot polymerize in the intracellular microenvironment. The polymerized Y-DLPA1 with red fluorescence provides a platform to label cells for long-term tracking studies. This polymerization reaction induced tumor cell apoptosis, increased cell viscosity and decreased cell motility, which potentially inhibited tumor metastasis and served as a novel antitumor agent. This work provides a novel strategy to track in-situ polymerization process and modulate cell biofunctions.
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