Journal
FRONTIERS IN CHEMISTRY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2022.871013
Keywords
biosensor; dual modes; mimic enzyme; hydrogen peroxide; glucose
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Mimic enzymes have gained attention in recent years due to their high stability, convenient preparation, and low price. This article presents the synthesis of Co3O4 nanosheets with peroxidase-like activity through a simple hydrothermal method. A colorimetric and fluorescence dual detection mode photochemical sensor based on the Co3O4 nanosheets is established for the detection of H2O2 and glucose. The sensor exhibits high sensitivity and strong anti-interference ability.
The mimic enzyme has become a research hotspot in recent years because of its advantages of high stability, convenient preparation, and low price. In this article, Co3O4 nanosheets synthesized by a simple hydrothermal method possess the characteristics of a peroxidase-like activity. The results demonstrated that 3,3 ',5,5 '-Tetramethylbenzidine (TMB) could be oxidized by H2O2 to produce a typical blue product (oxTMB) which has a strong absorption at 650 nm wavelength with the help of the Co3O4 nanosheets. Thus, a simple and sensitive colorimetric detection method for H2O2 was established with a good linear relationship (2-200 mu M) and a low limit of detection (0.4 mu M). Meanwhile, the colorimetric product can effectively quench the fluorescence emitted by Ru(bpy)(3)(2+). Therefore, a colorimetric and fluorescence dual detection mode photochemical sensor for H2O2 detection is constructed based on the principle of the inner filter effect (IFE) between the colorimetric product (oxTMB) and Ru(bpy)(3)(2+). It can effectively avoid the false positive problem of a single detection mode. In the presence of glucose oxidase, glucose can be catalyzed to produce gluconic acid and H2O2; therefore, the sensor can also be used for the determination of glucose with a good linear relationship (0.02-2 mu M) and a low limit of detection (5 nM). Experimental results showed that the sensor has a high sensitivity and strong anti-interference ability which can be used for the detection of actual samples.
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