Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 122, Issue 3, Pages 307-313Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2016.02.002
Keywords
Denitrification; Pseudomonas stutzeri; Acetogenesis; Sporomusa ovata; Co-culture; Solid-phase humin
Funding
- Ministry of Education, Culture, Sports, Science and Technology in Japan [26281040, 15K14904]
- Sekisui Chemical Grant Program for Research on Manufacturing Based on Innovations Inspired by Nature
- Asahi Glass Foundation
- Grants-in-Aid for Scientific Research [26281040, 15K14904] Funding Source: KAKEN
Ask authors/readers for more resources
A co-culture system comprising an acetogenic bacterium, Sporomusa ovata DSMZ2662, and a denitrifying bacterium, Pseudomonas stutzeri JCM20778, enabled denitrification using H-2 as the sole external electron donor and CO2 as the sole external carbon source. Acetate produced by S. ovata supported the heterotrophic denitrification of P. stutzeri. A nitrogen balance study showed the reduction of nitrate to nitrogen gas without the accumulation of nitrite and nitrous oxide in the co-culture system. S. ovata did not show nitrate reduction to ammonium in the co-culture system. Significant proportions of the consumed H-2 were utilized for denitrification: 79.9 +/- 4.6% in the co-culture system containing solid-phase humin and 62.9 +/- 11.1% in the humin-free co-culture system. The higher utilization efficiency of hydrogen in the humin-containing system was attributed to the higher denitrification activity of P. stutzeri under the acetate deficient conditions. The nitrogen removal rate of the humin-containing co-culture system reached 0.19 kg NO3--N.m(-3).d(-1). Stable denitrification activity for 61 days of successive sub-culturing suggested the robustness of this co-culture system. This study provides a novel strategy for the in situ enhancement of microbial denitrification. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available