Localized electrical stimulation triggers cell-type-specific proliferation in biofilms

Biological systems ranging from bacteria to mammals utilize electrochemical signaling. Although artificial electrochemical signals have been utilized to characterize neural tissue responses, the effects of such stimuli on non-neural systems remain unclear. To pursue this question, we developed an experimental platform that combines a microfluidic chip with a multielectrode array (MiCMA) to enable localized electrochemical stimulation of bacterial biofilms. The device also allows for the simultaneous measurement of the physiological response within the biofilm with single-cell resolution. We find that the stimulation of an electrode locally changes the ratio of the two major cell types comprising Bacillus subtilis biofilms, namely motile and extra cellular-matrix-producing cells. Specifically, stimulation promotes the proliferation of motile cells but not matrix cells, even though these two cell types are genetically identical and reside in the same microenvironment. Our work thus reveals that an electronic interface can selectively target bacterial cell types, enabling the control of biofilm composition and development.

Automated summary

This study developed an experimental platform combining a microfluidic chip with a multielectrode array to enable localized electrochemical stimulation of bacterial biofilms and simultaneous measurement of physiological response. The findings show that the stimulation of an electrode can selectively promote the proliferation of a specific bacterial cell type, revealing the potential control of biofilm composition and development through electronic interface.