Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 122, Issue 1, Pages 85-91Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2015.11.004
Keywords
Denitrification; Pseudomonas stutzeri; Bioelectrochemical system; Redox mediator; Solid-phase humin
Funding
- Ministry of Education, Culture, Sports, Science and Technology in Japan [23310055, 26281040]
- Sekisui Chemical Grant Program for Research on Manufacturing Based on Innovations Inspired by Nature
- Asahi Glass Foundation
- Grants-in-Aid for Scientific Research [26281040, 23310055] Funding Source: KAKEN
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The denitrification reactions performed by Pseudomonas stutzeri JCM20778 were enhanced electrochemically with the use of solid-phase humin, although P. stutzeri itself was incapable of receiving electrons directly from the graphite electrode. Electrochemically reduced humin enhanced the microbial, but not abiotic, denitrification reactions. Electric current and cyclic voltammetry analyses suggested that the solid-phase humin functioned as an electron donor for the denitrification reactions of P. stutzeri. Nitrogen balance study and the estimation of the first-order rate constants of the consecutive denitrification reactions suggested that the solid-phase humin enhanced all reducing reactions from nitrate to nitrogen gas. Considering the wide distribution of humin in the environment, the findings that solid-phase humin can assist in electron transfer, from the electrode to a denitrifying bacterium that has little ability to directly utilize external electrons, has important implications for the widespread application of bioelectrochemical systems assisted by solid-phase humin for enhancing microbial denitrification. (C) 2015 The Society for Biotechnology, Japan. All rights reserved.
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