Journal
JOURNAL OF BIOPHOTONICS
Volume 9, Issue 10, Pages 1077-1084Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201500315
Keywords
surface plasmon resonance; plastic optical fibre; C-reactive protein; serum; microfluidic system
Categories
Funding
- European Community [FP7-611682]
- [RBFR122KL1]
Ask authors/readers for more resources
A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable conditions during the measurements. A working range of 0.006-70 mg L-1 and a limit of detection of 0.009 mg L-1 were achieved. These results are among the best compared to other SPR-based biosensors for CRP detection, especially considering that they were achieved in a real and complex medium, i.e. serum. In addition, since the sensor performances satisfy those requested in physiologically-relevant clinical applications, the whole biosensing platform could well address high sensitive, easy to realize, real-time, label-free, portable and low cost diagnosis of CRP for future lab-on-a-chip applications. [GRAPHICS] 3D sketch (left) of the thermo-stabilized home-made flow cell developed to house the SPR-based plastic optical fibre biosensor. Exemplary response curve (shift of the SPR wavelength versus time) of the proposed biosensor (right) for the detection of C-reactive protein in serum.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available