Journal
JOURNAL OF BIOPHOTONICS
Volume 10, Issue 1, Pages 61-67Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201600154
Keywords
BiMW interferometer; silicon nanowaveguides; label-free biosensing; hGH detection; pg/mL detection range
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Funding
- Project EPISENS of Spanish Ministry of Science and Innovation [TEC2012-34280]
- HEPATOPOC project from CIBER-BBN
- Departament d'Universitats, Recerca i Societat de la Informacio de la Generalitat de Catalunya [2014 SGR 624]
- Severo Ochoa Centers of Excellence Program of Spanish MINECO [SEV-2013-0295]
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A label-free interferometric transducer showing a theoretical detection limit for homogeneous sensing of 5 x 10(-8) RIU, being equivalent to a protein mass coverage resolution of 2.8 fg mm(-2), is used to develop a high sensitive biosensor for protein detection. The extreme sensitivity of this transducer combined with a selective bioreceptor layer enables the direct evaluation of the human growth hormone (hGH) in undiluted urine matrix in the 10 pg mL(-1) range.
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