Journal
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
Volume 34, Issue 10, Pages 2146-2154Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2015.1109553
Keywords
binding capacity; molecular docking; lysozyme; thiamine; drug selective electrode
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The binding of thiamine (vitamin B-1) on lysozyme has been examined at various ionic strengths of phosphate buffer (pH 6.9), various pH values, and various protein concentrations at 25 degrees C using thiamine selective membrane electrode. This method is faster and more precise than equilibrium dialysis technique which can obtain sufficient and accurate data for binding analysis. The values of Hill equation parameters were estimated for each set using binding capacity concept and used for calculation of intrinsic binding affinity. The results represent two binding sets for thiamine on lysozyme at various experimental conditions.
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