Journal
BIOENGINEERED
Volume 13, Issue 3, Pages 6880-6894Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2022.2044274
Keywords
Human umbilical cord mesenchymal stem cell; long non-coding RNAs; osteogenic differentiation; gene expression
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Funding
- National Natural Science Foundation of China [81771049]
- Department of Science and Technology of Sichuan Province [2020YJ0228]
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This study investigated the expression and function of lncRNAs in hUCMSC osteogenic differentiation. A total of 343 differentially expressed lncRNAs were identified. Bioinformatic analysis showed their involvement in intracellular function and phosphatidylinositol signaling system pathway. The impact of lncRNA H19 on osteogenic differentiation was validated through the construction of competing endogenous RNA networks.
Human umbilical cord mesenchymal stem cells (hUCMSCs) are attractive therapeutic cells for tissue engineering to treat bone defects. However, how the cells can differentiate into bone remains unclear. Long non-coding RNAs (lncRNAs) are non-coding RNAs that participate in many biological processes, including stem cell differentiation. In this study, we investigated the profiles and functions of lncRNAs in the osteogenic differentiation of hUCMSCs. We identified 343 lncRNAs differentially expressed during osteogenic differentiation, of which 115 were upregulated and 228 were downregulated. We further analyzed these lncRNAs using bioinformatic analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. GO and KEGG pathway analysis showed that 'intracellular part' and 'Phosphatidylinositol signaling system' were the most correlated molecular function and pathway, respectively. We selected the top 10 upregulated lncRNAs to construct six competing endogenous RNA networks. We validated the impact of the lncRNA H19 on osteogenic differentiation by overexpressing it in hUCMSCs. Overall, our results pave the way to detailed studies of the molecular mechanisms of hUCMSC osteogenic differentiation, and they provide a new theoretical basis to guide the therapeutic application of hUCMSCs.
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