Journal
APPLIED SCIENCES-BASEL
Volume 12, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/app12073232
Keywords
extraction improvement; enzymolysis; pectinolytic enzymes; proteolytic enzymes; green chemistry
Categories
Funding
- Medical University of Lublin
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Enzyme-assisted extraction (EAE) is a technique that uses hydrolytic enzymes to degrade cell walls for easier dissolution of metabolites. It is considered an eco-friendly and cost-effective improvement in extraction methods and has shown promising applications in the recovery of bioactive metabolites.
Enzyme-assisted extraction (EAE) involves the use of hydrolytic enzymes for the degradation of the cell wall or other cell components. This supports the diffusion of the solvent into the plant or fungal material, leading to easier elution of its metabolites. This technique has been gaining increasing attention, as it is considered an eco-friendly and cost-effective improvement on classical or modern extraction methods. Its promising application in improving the recovery of different classes of bioactive metabolites (e.g., polyphenols, carotenoids, polysaccharides, proteins, components of essential oil, and terpenes) has been reported by many scientific papers. This review summarises information on the theoretical aspects of EAE (e.g., the components of the cell walls and the types of enzymes used) and the most recent discoveries in the effective involvement of enzyme-assisted extraction of natural products (plants, mushrooms, and animals) for nutraceutical and pharmaceutical applications.
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