Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 21, Issue 12, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.21.12.127005
Keywords
surface-enhanced Raman spectroscopy; aptamer; plasmonic nanoparticles; competitive binding assay; molecular diagnostics
Funding
- National Institute of Environmental Health Sciences of the National Institutes of Health [2R44ES022303, P30ES023512]
- EPSRC Engineering and Physical Sciences Research Council [EP/L014165/1]
- Whitaker International Foundation
- EPSRC [EP/L014165/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/L014165/1] Funding Source: researchfish
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Conjugation of aptamers and their corresponding analytes onto plasmonic nanoparticles mediates the formation of nanoparticle assemblies: molecularly bound nanoclusters that cause a measurable change in the colloid's optical properties. The optimization of a surface-enhanced Raman spectroscopy (SERS) competitive binding assay utilizing plasmonic target and magnetic probe nanoparticles for the detection of the toxin bisphenol-A (BPA) is presented. These assay nanoclusters were housed inside three types of optofluidic chips patterned with magnetically activated nickel pads, in either a straight or array pattern. Both Fe2O3 and Fe2CoO4 were compared as potential magnetic cores for the silver-coated probe nanoparticles. We found that the Ag@Fe2O3 particles were, on average, more uniform in size and more stable than Ag@Fe2CoO4, whereas the addition of cobalt significantly improved the collection time of particles. Using Raman mapping of the assay housed within the magnetofluidic chips, it was determined that a 1 x 5 array of 50 mu m square nickel pads provided the most uniform SERS enhancement of the assay (coefficient of variation similar to 25%) within the magnetofluidic chip. Additionally, the packaged assay demonstrated the desired response to BPA, verifying the technology's potential to translate magnetic nanoparticle assays into a user-free optical analysis platform. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
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