Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 12, Issue 1, Pages 154-164Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2016.2134
Keywords
Cationic Nanoparticles; Cytotoxicity; Opsonization; Polyethyleneimine; Iron Oxide; Silica; Fibroblasts; Endothelia
Funding
- NIH [HD007495, DK56338, CA125123]
- Dan L. Duncan Cancer Center
- John S. Dunn Gulf Coast Consortium for Chemical Genomics
- National Institute of Health [RC2GM092599, U54CA143837]
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [U54HD007495] Funding Source: NIH RePORTER
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [P30HD007495, T32HD007495] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [P30CA125123, U54CA143837] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [P30DK056338] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [RC2GM092599] Funding Source: NIH RePORTER
- Biotechnology and Biological Sciences Research Council [BB/N005163/1] Funding Source: researchfish
- BBSRC [BB/N005163/1] Funding Source: UKRI
Ask authors/readers for more resources
Functionalization of nanoparticles with cationic moieties, such as polyethyleneimine (PEI), enhances binding to the cell membrane; however, it also disrupts the integrity of the cell's plasma and vesicular membranes, leading to cell death. Primary fibroblasts were found to display high surface affinity for cationic iron oxide nanoparticles and greater sensitivity than their immortalized counterparts. Treatment of cells with cationic nanoparticles in the presence of incremental increases in serum led to a corresponding linear decrease in cell death. The surface potential of the nanoparticles also decreased linearly as serum increased and this was strongly and inversely correlated with cell death. While low doses of nanoparticles were rendered non-toxic in 25% serum, large doses overcame the toxic threshold. Serum did not reduce nanoparticle association with primary fibroblasts, indicating that the decrease in nanoparticle cytotoxicity was based on serum masking of the PEI surface, rather than decreased exposure. Primary endothelial cells were likewise more sensitive to the cytotoxic effects of cationic nanoparticles than their immortalized counterparts, and this held true for cellular responses to cationic microparticles despite the much lower toxicity of microparticles compared to nanoparticles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available