Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 12, Issue 11, Pages 1987-2000Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2016.2299
Keywords
Peripheral Nerve Regeneration; Electrospinning; NGF; GM1
Funding
- National Natural Science Foundation of China [31470941, 31271035]
- Science and Technology Commission of Shanghai Municipality [15JC1490100, 15441905100]
- Ph.D. Programs Foundation of Ministry of Education of China [20130075110005]
- light of textile project [J201404]
- Deanship of Scientific Research at King Saud University [RGP-201]
- China Guangdong Innovative Research Team Program [2011S055]
- China Shenzhen Peacock Plan Project [KQTD201209]
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Nerve growth factor (NGF) is widely used for repairing peripheral nerve injury because of its capability in dominating the survival, migration, proliferation, and differentiation of nerve cells. Monosialoganglioside (GM1), as another kind of nerve growth factor, works for regulating NGF function. In this study, GM1 and NGF were incorporated into the Poly(l-lactic acid-co- epsilon-caprolactone)/silk fibroin (PLCL/SF) nanofibers by the coaxial electrospinning. The fibers morphology and core-shell structure were characterized by SEM and TEM. The scaffolds demonstrated high tensile stress with good flexibility. In vitro cell viability studies indicated that the scaffolds incorporating both GM1 and NGF played a synergistic effect to enhance Schwann cells (SCs) proliferation and Pheochromocytoma (PC12) cells differentiation, in comparison to the scaffolds only incorporating NGF. Subsequently, the nanofibrous conduit scaffolds (NCSs) were evaluated in vivo in a rabbit sciatic nerve defect model. The NGF/GM1 incorporated NCSs group performed better nerve function recovery than single incorporated group, in consideration of the compound muscle action potential (CMAP) and nerve conduction velocity (NCV) results. Furthermore, hematoxylin and eosin (H&E) staining, toluidine blue (TB) staining, and transmission electron microscope (TEM) analysis displayed better nerve regeneration of NGF/GM1 incorporated NCSs both quantitatively and qualitatively. Therefore, the results indicated the dual neurotrophins-incorporated NCSs had potentials for the application in peripheral nerve repairing.
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