Journal
ADVANCED SCIENCE
Volume 9, Issue 16, Pages -Publisher
WILEY
DOI: 10.1002/advs.202106075
Keywords
epithelial and mesenchymal organoid; human induced pluripotent stem cells; localized scleroderma; proteomics; skin
Categories
Funding
- National Key Research and Development Program of China [2021YFA1301603]
- CAMS Innovation Fund for Medical Sciences (CIFMS) [2021-I2M-1-003]
- National Science and Technology Major Project [2018ZX09711003]
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The proteome map and pathological features of localized scleroderma (LoS) skin were established and characterized. It was found that the application of human-induced pluripotent stem cell-derived epithelial and mesenchymal (EM) organoids in a 3D culture system can reduce skin fibrosis and promote sweat gland and blood vessel regeneration.
Localized scleroderma (LoS) is a rare chronic disease with extensive tissue fibrosis, inflammatory infiltration, microvascular alterations, and epidermal appendage lesions. However, a deeper understanding of the pathogenesis and treatment strategies of LoS is currently limited. In the present work, a proteome map of LoS skin is established, and the pathological features of LoS skin are characterized. Most importantly, a human-induced pluripotent stem cell-derived epithelial and mesenchymal (EM) organoids model in a 3D culture system for LoS therapy is established. According to the findings, the application of EM organoids on scleroderma skin can significantly reduce the degree of skin fibrosis. In particular, EM organoids enhance the activity of epidermal stem cells in the LoS skin and promotes the regeneration of sweat glands and blood vessels. These results highlight the potential application of organoids for promoting the recovery of scleroderma associated phenotypes and skin-associated functions. Furthermore, it can provide a new therapeutic alternative for patients suffering from disfigurement and skin function defects caused by LoS.
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