Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 12, Issue 12, Pages 2172-2184Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2016.2318
Keywords
Mucin-1; Mesoporous Silica Nanoparticles; Diagnosis; Breast Cancer; Transgenic Mouse Model
Funding
- North Carolina Biotechnology Center (NCBC Loan) [2014-SRL-2604]
- Center for Biomedical and Engineering Sciences (CBES)
- UNC Charlotte
- Nanoscale Science Program at UNC Charlotte
- NIH AREA [1R15CA192160-01]
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Mucin-1 (MUC1), a transmembrane glycoprotein is aberrantly expressed on similar to 90% of breast cancer and is an excellent target for nanoparticulate targeted imaging. In this study, the development of a dye-doped NIR emitting mesoporous silica nanoparticles platform conjugated to tumor-specific MUC1 antibody (ab-tMUC1-NIR-MSN) for in vivo optical detection of breast adenocarcinoma tissue is reported. The structural properties, the in vitro and in vivo performance of this nanoparticle-based probe were evaluated. In vitro studies showed that the MSN-based optical imaging nanoprobe is non-cytotoxic and targets efficiently mammary cancer cells overexpressing human tMUC1 protein. In vivo experiments with female C57BL/6 mice indicated that this platform accumulates mainly in the liver and did not induce short-term toxicity. In addition, we demonstrated that the ab-tMUC1-NIR-MSN nanoprobe specifically detects mammary gland tumors overexpressing human tMUC1 in a human MUC1 transgenic mouse model.
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