Application of a Cascaded Nanozyme in Infected Wound Recovery of Diabetic Mice

The emergence of peroxidase (POD)-like nano-zyme-derived catalytic therapy has provided a promising choice forreactive oxygen species (ROS)-mediated broad-spectrum anti-bacterials to replace antibiotics, but it still suffers from limitationsof low therapeutic efficiency and unusual addition of unstableH2O2. Considering that the higher blood glucose in diabeticwounds provides much more numerous nutrients for bacterialgrowth, a cascade nanoenzymatic active material was developed bycoating glucose oxidase (GOx) onto POD-like Fe2(MoO4)3[Fe2(MoO4)3@GOx]. GOx could consume the nutrient of glucoseto produce gluconic acid (weakly acidic) and H2O2, which couldbe subsequently converted into highly oxidative center dot OH via thecatalysis of POD-like Fe2(MoO4)3. Accordingly, the synergisticeffect of starvation and ROS-mediated therapy showed significantly efficient antibacterial effect while avoiding the external additionof H2O2that affects the stability and efficacy of the therapy system. Compared with the bactericidal rates of 46.2-59.404% of GOxor Fe2(MoO4)3alone on extended-spectrum beta-lactamases producingEscherichia coliand methicillin-resistantStaphylococcus aureus,those of the Fe2(MoO4)3@GOx group are 98.396 and 98.776%, respectively. Animal experiments showed that the as-synthesizedFe2(MoO4)3@GOx could much efficiently promote the recovery of infected wounds in type 2 diabetic mice while showing lowcytotoxicityin vivo

Automated summary

The emergence of peroxidase (POD)-like nano-zyme-derived catalytic therapy has provided a promising choice for reactive oxygen species (ROS)-mediated broad-spectrum anti-bacterials to replace antibiotics. By coating glucose oxidase (GOx) onto POD-like Fe2(MoO4)3, a cascade nanoenzymatic active material was developed, which achieved the synergistic effect of starvation and ROS-mediated therapy, showing high antibacterial efficacy.