Journal
STEM CELL REPORTS
Volume 17, Issue 5, Pages 1170-1182Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2022.03.012
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- [19K18179]
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Dynamic culture of human-induced pluripotent stem cell-derived cardiomyocytes in a rotating wall vessel bioreactor significantly improves cellular function and muscle contraction and electrical properties of cardiac tissues, promoting the recovery of cardiac performance.
A rotating wall vessel (RWV) bioreactor was constructed for growing massive functional cardiac constructs to recover the function of a distressed rat heart. Three-dimensional cardiac tissues were engineered by seeding human-induced pluripotent stem cell-derived cardiomyocytes on poly(lactic-co-glycolic acid) fiber sheets (3D-hiPSC-CTs) and cultured in the RWV bioreactor (RWV group) or under static conditions (control group). The tissues were transplanted into a myocardial infarction nude rat model, and cardiac performance was evaluated. In the RWV group, cell viability and contractile and electrical properties significantly improved, mature cardiomyocytes were observed, and mechanical stress-related mediators of mammalian target of rapamycin signaling were upregulated compared with those of the control. Four weeks post-transplantation, tissue survival and left ventricular ejection fraction significantly improved in the RWV group. Hence, dynamic culture in an RWV bioreactor could provide a superior culture environment for improved performance of 3DhiPSC-CTs, providing a means for functional cardiomyogenesis in myocyte-loss heart failure.
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