Journal
NANOMATERIALS
Volume 12, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/nano12101692
Keywords
magnetic nanoparticles; fluorination; polyethyleneimine; polyethylene glycol; RNA interference; CXC chemokine receptor 4
Categories
Funding
- National Key Research and Development Program of China [2017 YFA0205502]
- National Natural Science Foundation of China [82072067, 61821002]
- Special Fund for Transformation of Scientific and Technological Achievements of Jiangsu Province [BA2020016]
- Fundamental Research Funds for the Central Universities
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In this study, we successfully prepared Fluorinated Cationic Polymer-Coated Magnetic Nanoparticles (FPP@MNPs) as carriers for siRNA. It was demonstrated that FPP@MNPs have good biocompatibility and high cellular uptake efficiency, and can successfully transfect cells and reduce the expression of CXCR4.
CXC chemokine receptor 4 (CXCR4) is a promising therapeutic target. Previous studies have shown that intracellular delivery of siRNA to knockdown CXCR4 expression in cancer cells is an effective therapeutic strategy. To prepare efficient magnetic nucleic acid carriers, it is now necessary to improve the endocytosis efficiency of PEGylated magnetic nanoparticles. In our work, Heptafluorobutyryl-polyethylene glycol-polyethyleneimine (FPP) was first prepared and then used to coat magnetic nanoparticles (MNPs) to obtain magnetic nanocarriers FPP@MNPs. The materials were characterized by (19) F-Nuclear Magnetic Resonance (NMR), transmission electron microscope (TEM), energy dispersive spectroscopy (EDS), and dynamic light scattering (DLS). The biosecurity of FPP@MNPs was confirmed by cell viability and apoptosis experiments. Cellular uptake of FPP@MNPs and siRNA transfection enhanced by external magnetic fields were detected by fluorescence microscopy, confocal laser microscopy, and flow cytometry. The results show that the cellular uptake efficiency of FPP@MNPs was significantly improved, and transfection efficiency reached more than 90%. The knockdown of CXCR4 on the 4 T1 cell membrane was confirmed by real-time polymerase chain reaction (RT-PCR) and flow cytometry. In conclusion, the fluorinated cationic polymer-coated magnetic nanoparticles FPP@MNPs can be loaded with siRNA to reduce CXCR4 expression as well as be expected to be efficient universal siRNA carriers.
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