Journal
ACS PHOTONICS
Volume 9, Issue 3, Pages 1042-1049Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsphotonics.1c02015
Keywords
wide-field microscopy; surface-enhanced coherent anti-Stokes Raman scattering; hyperspectral imaging 3D live-cell imaging; label freebiomolecular imaging
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Funding
- NIH [R35 GM136223, R01 AI141439]
- NSF [CHE1807106]
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This study introduces a wide-field surface-enhanced coherent anti-Stokes Raman scattering (WISE-CARS) microscope for monitoring nanotags in live cells and label-free detection of metabolic molecules. The microscope achieves high imaging speed and resolution for spectral focusing of femtosecond lasers, allowing for rapid hyperspectral imaging of over 1 million Raman spectra.
Surface-enhanced Raman scattering (SERS) spectroscopy has been used extensively to study biology, chemistry, and materials. However, point-by-point SERS mapping is time-consuming, taking minutes to hours for large-scale imaging. Here, we report a wide-field surface-enhanced coherent anti-Stokes Raman scattering (WISE-CARS) microscope for monitoring nanotags in live cells and label-free detection of metabolic molecules. The WISE-CARS microscope achieves an imaging speed of 120 fps for a field of view of 130 mu m x 130 mu m. By spectral focusing of femtosecond lasers, a hyperspectral WISE-CARS stack of 60 frames can be acquired within 0.5 s, where over 1 million Raman spectra are parallelly recorded with a spectral resolution of 10 cm(-1). As applications, we demonstrate time-lapse, three-dimensional (3D) WISE-CARS imaging of nanotags in live cells as well as label-free detection of adenine released from S. aureus.
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