Journal
JOURNAL OF BIOMATERIALS APPLICATIONS
Volume 31, Issue 2, Pages 181-192Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328216652537
Keywords
Electrohydrodynamic jet 3D printing; polycaprolactone; chitosan; tissue engineering; biomaterials; human embryonic stem cells; fibroblasts
Funding
- National University of Singapore Research Scholarship
- NUS Research Scholarship
- President Graduate Fellowship
Ask authors/readers for more resources
Biological function of adherent cells depends on the cell-cell and cell-matrix interactions in three-dimensional space. To understand the behavior of cells in 3D environment and their interactions with neighboring cells and matrix requires 3D culture systems. Here, we present a novel 3D cell carrier scaffold that provides an environment for routine 3D cell growth invitro. We have developed thin, mechanically stable electrohydrodynamic jet (E-jet) 3D printed polycaprolactone and polycaprolactone/Chitosan macroporous scaffolds with precise fiber orientation for basic 3D cell culture application. We have evaluated the application of this technology by growing human embryonic stem cell-derived fibroblasts within these 3D scaffolds. Assessment of cell viability and proliferation of cells seeded on polycaprolactone and polycaprolactone/Chitosan 3D-scaffolds show that the human embryonic stem cell-derived fibroblasts could adhere and proliferate on the scaffolds over time. Further, using confocal microscopy we demonstrate the ability to use fluorescence-labelled cells that could be microscopically monitored in real-time. Hence, these 3D printed polycaprolactone and polycaprolactone/Chitosan scaffolds could be used as a cell carrier for invitro 3D cell culture-, bioreactor- and tissue engineering-related applications in the future.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available