Journal
LIGHT-SCIENCE & APPLICATIONS
Volume 11, Issue 1, Pages -Publisher
SPRINGERNATURE
DOI: 10.1038/s41377-022-00763-2
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Sequential excitation technique with minimal light enables precise localization of single fluorescent molecules with molecular resolution. Expanding this concept to multi-photon regimes can lead to even higher localization precision and deeper imaging in biological specimens.
Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1-2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.
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