Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 291, Issue 43, Pages 22671-22685Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M116.739573
Keywords
mRNA; post-translational modification (PTM); protein arginine N-methyltransferase 5 (PRMT5); RNA binding protein; stress granule; G3BP1; protein arginine N-methyltransferase (PRMT)
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Funding
- National Institutes of Health Public Health Service Grant [AI50237]
- NCI Cancer Center Support Grant [P30CA125123]
- Integrated Microscopy Core at Baylor College of Medicine
- National Institutes of Health [HD007495, DK56338, CA125123]
- Dan L. Duncan Cancer Center
- John S. Dunn Gulf Coast Consortium for Chemical Genomics
- National Institutes of Health funding [DK062248]
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Stress granules (SGs) are cytoplasmic condensates of stalled messenger ribonucleoprotein complexes (mRNPs) that form when eukaryotic cells encounter environmental stress. RNA-binding proteins are enriched for arginine methylation and facilitate SG assembly through interactions involving regions of low amino acid complexity. How methylation of specific RNA-binding proteins regulates RNA granule assembly has not been characterized. Here, we examined the potent SG-nucleating protein Ras-GAP SH3-binding protein 1 (G3BP1), and found that G3BP1 is differentially methylated on specific arginine residues by protein arginine methyltransferase (PRMT) 1 and PRMT5 in its RGG domain. Several genetic and biochemical interventions that increased methylation repressed SG assembly, whereas interventions that decreased methylation promoted SG assembly. Arsenite stress quickly and reversibly decreased asymmetric arginine methylation on G3BP1. These data indicate that arginine methylation in the RGG domain prevents large SG assembly and rapid demethylation is a novel signal that regulates SG formation.
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