Journal
FRONTIERS IN PLANT SCIENCE
Volume 13, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2022.870695
Keywords
GmSOS1; soybean; Na+ efflux; salt tolerance; breeding
Categories
Funding
- National Natural Science Foundation of China [31801444]
- Heilongjiang Provincial Natural Science Foundation of China [LH2021C005]
- Heilongjiang postdoctoral Fund [LBH-Z14009]
- National Non-Profit Institute Research Grant of the Chinese Academy of Forestry [CAFYBB2019ZY003]
- Fundamental Research Funds for the Central Universities [2572020DP01]
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In this study, gmsos1 mutants were created using the CRISPR-Cas9 system in soybean, revealing the critical role of GmSOS1 in maintaining Na+ homeostasis for salt tolerance in soybean. The accumulation of Na+ in the mutant roots resulted in an imbalance between Na+ and K+, which inhibited Na+ efflux and increased K+ efflux.
Soybean (Glycine max) is a staple crop and a major source of vegetable protein and vegetable oil. The growth of soybean is dramatically inhibited by salt stress, especially by the excessive toxic Na+. Salt Overly Sensitive 1 (SOS1) is the only extensively characterized Na+ efflux transporter in multiple plant species so far. However, the role of GmSOS1 in soybean salt stress responses remains unclear. Herein, we created three gmsos1 mutants using the CRISPR-Cas9 system in soybean. We found a significant accumulation of Na+ in the roots of the gmsos1 mutants, resulting in the imbalance of Na+ and K+, which links to impaired Na+ efflux and increased K+ efflux in the roots of the gmsos1 mutants under salt stress. Compared to the wild type, our RNA-seq analysis revealed that the roots of the gmsos1-1 showed preferential up and downregulation of ion transporters under salt stress, supporting impaired stress detection or an inability to develop a comprehensive response to salinity in the gmsos1 mutants. Our findings indicate that the plasma membrane Na+/H+ exchanger GmSOS1 plays a critical role in soybean salt tolerance by maintaining Na+ homeostasis and provides evidence for molecular breeding to improve salt tolerance in soybean and other crops.
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