Journal
FRONTIERS IN MICROBIOLOGY
Volume 13, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.814448
Keywords
prebiotic; AHCC (R); Pseudomonas aeruginosa; motility and biofilm; secretion system and adhesion; immune response; PCR real time (qPCR); internalization
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Funding
- FEDER project of Junta de Andalucia, Spain [30B572F301]
- Ministry of Economy and Competitivity
- Fondo Europeo de Desarrollo Regional FEDER funds [SAF2017-88457-R, AGL2017-85270-R]
- University of the Ministry of Education (Spain)
- Junta de Andalucia [CTS235, CTS164]
- Instituto de Salud Carlos III
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In this study, the potential antibacterial effect of a standardized extract of cultured mycelium of Lentinula edodes (AHCC (R)) on Pseudomonas aeruginosa was explored. The results showed that AHCC (R) can inhibit the growth rate and biofilm formation of strain PAO1, hamper swimming and twitching motility, decrease the expression of metabolism, growth, and biofilm formation genes, diminish the levels of exotoxin A and bacteria inside host cells, and reduce the secretion of certain cytokines. This suggests that AHCC (R) has a potential role in preventing P. aeruginosa infections and may lead to the development of new therapies.
The priority pathogen list of the World Health Organization classified Pseudomonas aeruginosa as the second top critical pathogen. Hence, the development of novel antibacterial strategies to tackle this bacterium is highly necessary. Herein we explore the potential antibacterial effect of a standardized extract of cultured mycelium of Lentinula edodes (AHCC (R)) on P. aeruginosa. AHCC (R) was found to inhibit the growth rate and biofilm formation of strain PAO1. No change in swarming was observed, but AHCC (R) hampered swimming and twitching motility. In accordance, a decreased expression of metabolism, growth, and biofilm formation genes was shown. AHCC (R) also diminished the levels of exotoxin A and bacteria inside IEC18 cells and the secretion of IL-6, IL-10 and TNF by infected macrophages. This effect was related to a reduced phosphorylation of MAPKs and to bacteria internalization. Taken together, our data suggest that AHCC (R) has a potential role to prevent P. aeruginosa infections and may lead to the development of new therapies.
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