Methanotrophy Alleviates Nitrogen Constraint of Carbon Turnover by Rice Root-Associated Microbiomes

The bioavailability of nitrogen constrains primary productivity, and ecosystem stoichiometry implies stimulation of N-2 fixation in association with carbon sequestration in hotspots such as paddy soils. In this study, we show that N-2 fixation was triggered by methane oxidation and the methanotrophs serve as microbial engines driving the turnover of carbon and nitrogen in rice roots. N-15(2)-stable isotope probing showed that N-2-fixing activity was stimulated 160-fold by CH4 oxidation from 0.27 to 43.3 mu mol N g(-1) dry weight root biomass, and approximately 42.5% of the fixed N existed in the form of N-15-NH4+ through microbial mineralization. Nitrate amendment almost completely abolished N-2 fixation. Ecophysiology flux measurement indicated that methane oxidation-induced N-2 fixation contributed only 1.9% of total nitrogen, whereas methanotrophy-primed mineralization accounted for 21.7% of total nitrogen to facilitate root carbon turnover. DNA-based stable isotope probing further indicated that gammaproteobacterial Methylomonas-like methanotrophs dominated N-2 fixation in CH4-consuming roots, whereas nitrate addition resulted in the shift of the active population to alphaproteobacterial Methylocystis-like methanotrophs. Co-occurring pattern analysis of active microbial community further suggested that a number of keystone taxa could have played a major role in nitrogen acquisition through root decomposition and N-2 fixation to facilitate nutrient cycling while maintaining soil productivity. This study thus highlights the importance of root-associated methanotrophs as both biofilters of greenhouse gas methane and microbial engines of bioavailable nitrogen for rice growth.

Automated summary

The bioavailability of nitrogen for rice growth is constrained by primary productivity, and this study shows that methane oxidation triggers nitrogen fixation in rice roots, with methanotrophs playing a vital role in carbon and nitrogen turnover. The results highlight the significance of root-associated methanotrophs in providing bioavailable nitrogen for rice growth and their role as microbial engines of nutrient cycling.