4.7 Article

Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

Point-of-care diagnosis of invasive non-typhoidal Salmonella enterica in bloodstream infections using immunomagnetic capture and loop-mediated isothermal amplification

Aaydha C. Vinayaka et al.

Summary: Invasive non-typhoidal salmonellosis is gaining attention as an emerging disease cluster, particularly among immunocompromised and malnourished children. Point-of-care diagnostics hold promise for use in low infrastructure laboratory settings, but face challenges in dealing with the complexity of blood matrices.

NEW BIOTECHNOLOGY (2022)

Article Medical Laboratory Technology

Rapid and Extraction-Free Detection of SARS-CoV-2 from Saliva by Colorimetric Reverse-Transcription Loop-Mediated Isothermal Amplification

Matthew A. Lalli et al.

Summary: The study developed a rapid colorimetric assay for SARS-CoV-2 detection using saliva samples without RNA extraction, achieving high accuracy and sensitivity.

CLINICAL CHEMISTRY (2021)

Article Biochemical Research Methods

An in-well direct lysis method for rapid detection of SARS-CoV-2 by real time RT-PCR in eSwab specimens

Rikke Lind Jorgensen et al.

Summary: By adding detergent to the rRT-PCR master mix, it is possible to detect SARS-CoV-2 directly in eSwab samples without the need for NA purification. The study results showed that direct lysis rRT-PCR detected SARS-CoV-2 in 27 out of 30 previously identified positive samples, demonstrating high accuracy.

JOURNAL OF VIROLOGICAL METHODS (2021)

Article Infectious Diseases

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Victor M. Corman et al.

EUROSURVEILLANCE (2020)

Article Medicine, General & Internal

A Novel Coronavirus from Patients with Pneumonia in China, 2019

Na Zhu et al.

NEW ENGLAND JOURNAL OF MEDICINE (2020)

Article Multidisciplinary Sciences

SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification

Brian A. Rabe et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Biochemical Research Methods

Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes

Nathan A. Tanner et al.

BIOTECHNIQUES (2015)

Article Engineering, Environmental

DNA Extraction-Free Quantification of Dehalococcoides spp. in Groundwater Using a Hand-Held Device

Robert D. Stedtfeld et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2014)

Editorial Material Infectious Diseases

Loop-Mediated Isothermal Amplification Technology: Towards Point of Care Diagnostics

Zablon Kithinji Njiru

PLOS NEGLECTED TROPICAL DISEASES (2012)

Article Immunology

Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications

Patrice Francois et al.

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY (2011)

Article Biochemical Research Methods

Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products

Norihiro Tomita et al.

NATURE PROTOCOLS (2008)

Review Infectious Diseases

False-positive results and contamination in nucleic acid amplification assays: Suggestions for a prevent and destroy strategy

A Borst et al.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2004)

Article Biochemical Research Methods

Real-time turbidimetry of LAMP reaction for quantifying template DNA

Y Mori et al.

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS (2004)

Article Biochemical Research Methods

Accelerated reaction by loop-mediated isothermal amplification using loop primers

K Nagamine et al.

MOLECULAR AND CELLULAR PROBES (2002)

Article Biochemistry & Molecular Biology

Loop-mediated isothermal amplification of DNA

Tsugunori Notomi et al.

NUCLEIC ACIDS RESEARCH (2000)