Journal
ELIFE
Volume 11, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.71196
Keywords
aging; DNA circles; NPC; nuclear basket; S; cerevisiae
Categories
Funding
- Schweizerischer Nationalfonds zur Foerderung der Wissenschaftlichen Forschung [31,003 A-105904]
- H2020 European Research Council [31003A-105904]
- [899417]
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The attachment of DNA circles to NPCs causes NPCs to lose their nuclear and cytoplasmic complexes in replicatively ageing yeast cells. This process is related to the replicative lifespan of the cells.
The nuclear pore complex (NPC) mediates nearly all exchanges between nucleus and cytoplasm, and in many species, it changes composition as the organism ages. However, how these changes arise and whether they contribute themselves to ageing is poorly understood. We show that SAGA-dependent attachment of DNA circles to NPCs in replicatively ageing yeast cells causes NPCs to lose their nuclear basket and cytoplasmic complexes. These NPCs were not recognized as defective by the NPC quality control machinery (SINC) and not targeted by ESCRTs. They interacted normally or more effectively with protein import and export factors but specifically lost mRNA export factors. Acetylation of Nup60 drove the displacement of basket and cytoplasmic complexes from circle-bound NPCs. Mutations preventing this remodeling extended the replicative lifespan of the cells. Thus, our data suggest that the anchorage of accumulating circles locks NPCs in a specialized state and that this process is intrinsically linked to the mechanisms by which ERCs promote ageing.
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