Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 291, Issue 27, Pages 13987-13998Publisher
ELSEVIER
DOI: 10.1074/jbc.M116.725739
Keywords
adaptor protein; cell invasion; cell migration; focal adhesion; Rho (Rho GTPase); FHL2; mAbp1
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Funding
- National Institutes of Health [T32 CA009135, R01 CA085862]
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Mammalian actin-binding protein-1 (mAbp1) is an adaptor protein that binds actin and modulates scission during endocytosis. Recent studies suggest that mAbp1 impairs cell invasion; however, the mechanism for the inhibitory effects of mAbp1 remain unclear. We performed a yeast two-hybrid screen and identified the adaptor protein, FHL2, as a novel binding partner that interacts with the N-terminal actin depolymerizing factor homology domain (ADFH) domain of mAbp1. Here we report that depletion of mAbp1 or ectopic expression of the ADFH domain of mAbp1 increased Rho GTPase signaling and breast cancer cell invasion. Moreover, cell invasion induced by the ADFH domain of mAbp1 required the expression of FHL2. Taken together, our findings show that mAbp1 and FHL2 are novel binding partners that differentially regulate Rho GTPase signaling and MTLn3 breast cancer cell invasion.
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