Journal
CANCER LETTERS
Volume 366, Issue 2, Pages 182-190Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2015.06.018
Keywords
Gene expression; GFP; Histological analysis; MES-SA; PET imaging
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology [25861481]
- Ministry of Health, Labour and Welfare
- Smoking Research Foundation
- Grants-in-Aid for Scientific Research [25861481, 25861482] Funding Source: KAKEN
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Uterine sarcomas are rare and aggressive gynecologic tumors with a poor prognosis because of recurrence and metastasis. However, the mechanisms of uterine sarcoma metastasis are largely unknown. To investigate this mechanism, we developed a novel uterine sarcoma tissue-derived orthotopic and metastatic model in KSN nude mice using a green fluorescent protein stably expressed uterine sarcoma cell line, MES-SA. Histological analysis showed that all orthotopic primary tumors were undifferentiated sarcoma. Primary tumors were characterized by high F-18-fluorodeoxyglucose uptake with a positive correlation to the number of pulmonary metastases. In addition, we generated uterine sarcoma cell sublines with high or low metastatic potentials by serial in vivo selection. Microarray analysis between orthotopic tumors with high and low metastatic potentials revealed differential expression of genes related to cell proliferation and migration (TNNT1, COL1A2, and ZIC1). Our model would be useful to compensate for the limited clinical cases of uterine sarcoma and to investigate the molecular mechanisms of metastatic uterine sarcoma. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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