Journal
STEM CELLS TRANSLATIONAL MEDICINE
Volume 11, Issue 6, Pages 659-673Publisher
OXFORD UNIV PRESS
DOI: 10.1093/stcltm/szac025
Keywords
periostin; urothelial regeneration; cystitis; macrophage polarization; biomaterials
Categories
Funding
- National Key Research and Development Program of China [2017YFA0103802]
- National Natural Science Foundation of China [31972894, 81770290, 81671449, 81971314]
- Science and Technology Planning Project of Guangdong Province [2016B030229006, 2014A020211007, 2016B030230001]
- Key Scientific and Technological Program of Guangzhou City [201604020158, 201604020189]
- Key Program of Natural Science Foundation of Guangdong Province [2018B030311039]
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Postn plays an important role in bladder urothelial regeneration by inducing Wnt4 upregulation and AKT signaling activation, leading to urothelial stem cell proliferation. It also promotes resident macrophage proliferation and polarization to a pro-regenerative phenotype. Injectable P-GelMA granular hydrogel can deliver recombinant POSTN into the bladder and alleviate interstitial cystitis symptoms.
Interstitial cystitis (IC) is a bladder syndrome of unclear etiology with no generally accepted treatment. Growing evidence suggest that periostin (POSTN) is an important homeostatic component in the tissue repair and regeneration in adulthood, but its function in urinary bladder regeneration is still unknown. Here we investigate whether POSTN is involved in bladder tissue repair in a cyclophosphamide (CYP)-induced interstitial cystitis model. POSTN is primarily expressed in bladder stroma (detrusor smooth muscle and lamina propria) and upregulated in response to CYP-induced injury. POSTN deficiency resulted in more severe hematuria, aggravated edema of the bladder, and delayed umbrella cell recovery. Besides, less proliferative urothelial cells (labeled by pHH3, Ki67, and EdU) and lower expression of Krt14 (a urothelial stem cell marker) were detected in POSTN-/- mice post CYP exposure, indicating a limited urothelial regeneration. Further investigations revealed that POSTN could induce Wnt4 upregulation and activate AKT signaling, which together activates beta-catenin signaling to drive urothelial stem cell proliferation. In addition, POSTN can promote resident macrophage proliferation and polarization to a pro-regenerative (M2) phenotype, which favors urothelial regeneration. Furthermore, we generated injectable P-GelMA granular hydrogel as a biomaterial carrier to deliver recombinant POSTN into the bladder, which could increase urothelial stem cells number, decrease umbrella cells exfoliation, and hence alleviate hematuria in a CYP-induced interstitial cystitis model. In summary, our findings identify a pivotal role of POSTN in bladder urothelial regeneration and suggest that intravesical biomaterials-assisted POSTN delivery may be an efficacious treatment for interstitial cystitis.
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