First Form, Then Function: 3D Reconstruction of Cucumber Plants (Cucumis sativus L.) Allows Early Detection of Stress Effects through Leaf Dimensions

Detection of morphological stress symptoms through 3D examination of plants might be a cost-efficient way to avoid yield losses and ensure product quality in agricultural and horticultural production. Although the 3D reconstruction of plants was intensively performed, the relationships between morphological and physiological plant responses to salinity stress need to be established. Therefore, cucumber plants were grown in a greenhouse in nutrient solutions under three salinity treatments: 0, 25, and 50 mM NaCl. To detect stress-induced changes in leaf transversal and longitudinal angles and dimensions, photographs were taken from plants for 3D reconstruction through photogrammetry. For assessment of physiological stress responses, invasive leaf measurements, including the determination of leaf osmotic potential, leaf relative water content, and the leaf dry to fresh weight ratio, were performed. The transversal and longitudinal leaf dimensions revealed statistically significant differences between stressed and control plants after 60 degrees Cd (day 3) for the leaves which appeared before stress imposition. Strong correlations were found between the transversal width and some investigated physiological traits. Morphological changes were shown as indicators of physiological responses of leaves under salinity stress.

Automated summary

Detection of morphological stress symptoms through 3D examination of plants is a cost-efficient way to prevent yield losses and ensure product quality in agriculture and horticulture. The relationship between morphological and physiological plant responses to salinity stress needs further investigation. The study showed that morphological changes in leaf dimensions can serve as indicators for physiological responses in leaves under salinity stress.