Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276

Biosurfactants synthesized by microorganisms represent safe and sustainable alternatives to the use of synthetic surfactants, due to their lower toxicity, better biodegradability and biocompatibility, and their production from low-cost feedstocks. In line with this, the present study describes the physical, chemical, and functional characterization of the biopolymer secreted by the bacterium Burkholderia thailandensis DSM 13276, envisaging its validation as a biosurfactant. The biopolymer was found to be a glycolipopeptide with carbohydrate and protein contents of 33.1 +/- 6.4% and 23.0 +/- 3.2%, respectively. Galactose, glucose, rhamnose, mannose, and glucuronic acid were detected in the carbohydrate moiety at a relative molar ratio of 4:3:2:2:1. It is a high-molecular-weight biopolymer (1.0 x 10(7) Da) with low polydispersity (1.66), and forms aqueous solutions with shear-thinning behavior, which remained after autoclaving. The biopolymer has demonstrated a good emulsionstabilizing capacity towards different hydrophobic compounds, namely, benzene, almond oil, and sunflower oil. The emulsions prepared with the biosurfactant, as well as with its autoclaved solution, displayed high emulsification activity (>90% and similar to 50%, respectively). Moreover, the almond and sunflower oil emulsions stabilized with the biosurfactant were stable for up to 4 weeks, which further supports the potential of this novel biopolymer for utilization as a natural bioemulsifier.

Automated summary

Biosurfactants synthesized by microorganisms are safe and sustainable alternatives to synthetic surfactants. This study characterizes a biopolymer secreted by Burkholderia thailandensis, highlighting its potential as a biosurfactant. The biopolymer is a glycolipopeptide with carbohydrate and protein contents of 33.1% and 23.0%, respectively. It displays good emulsion-stabilizing capacity and can form stable emulsions with hydrophobic compounds.