Journal
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
Volume 19, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/ijerph19105848
Keywords
antibiotic resistance; carbapenemases; Enterobacterales; urban aquatic environments
Funding
- FCT/MCTES through national funds [UIDP/50017/2020+UIDB/50017/2020 + LA/P/0094/2020, UIDB/04004/2020, SFRH/BD/132046/2017, CEECIND/00977/2020]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/132046/2017] Funding Source: FCT
Ask authors/readers for more resources
We assessed the proportion of carbapenem-resistant Enterobacterales (CRE) in urban ponds and identified 23 isolates. Our findings confirmed that urban ponds serve as reservoirs and dispersal sites for CRE, and revealed the presence of multiple carbapenemase-encoding genes.
Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant Enterobacterales (CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S rDNA sequencing, antibiotic susceptibility tests, detection of carbapenemase-encoding genes, and conjugation assays. Isolates were affiliated with Klebsiella (n = 1), Raoultella (n = 11), Citrobacter (n = 8), and Enterobacter (n = 3). Carbapenemase-encoding genes were detected in 21 isolates: bla(KPC) (n = 20), bla(GES-5) (n = 6), and bla(VIM) (n = 1), with 7 isolates carrying two carbapenemase genes. Clonal isolates were collected from different ponds and in different campaigns. Citrobacter F6, Raoultella N9, and Enterobacter N10 were predicted as pathogens from whole-genome sequence analysis, which also revealed the presence of several resistance genes and mobile genetic elements. We found that bla(KPC-3) was located on Tn4401b (Citrobacter F6 and Enterobacter N10) or Tn4401d (Raoultella N9). The former was part of an IncFIA-FII pBK30683-like plasmid. In addition, bla(GES-5) was in a class 3 integron, either chromosomal (Raoultella N9) or plasmidic (Enterobacter N10). Our findings confirmed the role of urban ponds as reservoirs and dispersal sites for CRE.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available